PREPARATION OF EXTRACTS.
نویسندگان
چکیده
منابع مشابه
Preparation of Retinal Extracts
By cannulating the stroma of rabbit corneae, and introducing extracts of retina and vitreous from newborn kittens subjected to vaso-obliterative doses of oxygen, an attempt was made to test the hypothesis that ischaemic retinal tissue releases a factor with vasoformative properties. It was found that, although retino-vitreal extracts from the test animals elicited a positive response in just ov...
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HeLa cells are the archetypal tissue culture cell line for the preparation of mammalian cell-free systems. These cells, derived from a cervical carcinoma, have been maintained in culture since the late 1940s. They grow with a doubling time of ∼24 h and can be cultured in medium containing fetal bovine serum (optimal serum for growth) or medium containing horse serum (in which they grow more slo...
متن کاملPreparation of extracts from animal tissues.
The initial procedure in the isolation of an protein, a protein complex, or a subcellular organelle is the preparation of an extract that contains the required component in a soluble form. Indeed, when undertaking a proteomic study, the production of a suitable cellular extract is essential. Further isolation of subcellular fractions depends on the ability to rupture the animal tissues in such ...
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For the preparation of lipide extracts from tissues, the method of Bloor (I), either in its original form or with slight modifications, has been a standard procedure. This method consists in extracting the tissue with a mixture of ethyl alcohol and ether. Since the extract obtained is known to contain non-lipide contaminants, it is usually taken to dryness and the residue extracted with a solve...
متن کاملPreparation of Lipid Extracts from Tissues
A. Introduction B. Storage of Tissues and Preliminary Treatments prior to Extraction C. Extraction of Tissues with Solvents D. Removal of Non-Lipid Contaminants from Extracts E. Artefacts of Extraction Procedures F. Practical Extraction Procedures 1. Some general comments 2. The "Folch" procedure 3. The Bligh and Dyer method 4. Extraction of plant tissues G. Some Special Cases 1. Acyl-coenzyme ...
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ژورنال
عنوان ژورنال: The Lancet
سال: 1833
ISSN: 0140-6736
DOI: 10.1016/s0140-6736(02)94925-1